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Western Blot Cell lysates were prepared using RIPA lysis buffer (Beyotime, P0013B) supplemented with 1% protease inhibitor cocktail (Cwbiotech, CW2200S) and 1% phosphatase inhibitor cocktail (Cwbiotech, CW2383S)
1068 Metformin also reduces insulin-like growth factor (IGF) signaling pathway activity, decreases glucose absorption, and promotes glucose breakdown, thus reducing energy supply and growth of cancer cells
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Inhibitory Mechanism of Melanin Synthesis by Glutathione. YAKUGAKU ZASSHI , vol