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Referral to specialist weight management services for intensive behavioural interventions, very-low-calorie diets, or consideration for bariatric surgery Bariatric surgery remains the most effective intervention for significant, sustained weight loss

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Arch Intern Med, 2006

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The cells were then centrifuged at 10,000 g for 2 min and incubated with 1 ml of 2 M HCl and 0.5% Triton X-100 (Sigma-Aldrich, 93443) for 30 min with gentle agitation