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Ghrelin receptor (GHS-R1a) activation by Ipamorelin couples primarily to Gq proteins, activating phospholipase C to generate IP and diacylglycerol

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coli , we reduced the reported disulfide bond between the active site cysteine C13 and GSH in the clarified cell lysate using dithiothreitol (DTT) and removed the reduced GSH with an extended on-column washing step during purification

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