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One microgram of total RNA from each sample was reverse transcribed, and the real-time RT-PCR measurements were performed, as described previously [49], using an apparatus Aria MX (Agilent Technologies, Santa Clara, CA, USA) with the corresponding SYBR Green kit, according to the Promega manufacturers recommendations

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Verification of PCOS induction Vaginal smears The stages of estrus were identified through microscopic examination of vaginal smears, which were collected daily from the start of the experiment until its conclusion

For physiological reasons, dietary vitamin B12 generally cannot cause hypercobalaminemia

Ezek nem trtak fel nagy aggodalomra okot ad mellkhatst, br a kutatk azt is megjegyeztk, hogy tovbbi vizsglatokra van szksg a biztonsgossg megllaptsra