Intracellular glutathione is synthesized in two enzymatic steps
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Type C possesses an epoxide ring at C8 while Type D trichothecenes possess a di-or tri-ester linkage spanning the C4 and C15 regions (Shank et al., 2011
in a 40:1 ratio
a , We constructed a mutant strain that could not synthesize glutathione by knocking out, in the wild-type strain, the GSH1 gene which is essential for glutathione biosynthesis (see Mutant yeasts in the Methods section)