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Treatment of hair follicle melanocytes in culture Prior to treatment, primary HFMs were incubated for 24 h in starved 2:1 melanocyte media (2:1 melanocyte media minus 1% FBS and BPE)

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In the lung, alveolar type II epithelial cells can synthesize DA, which attenuates lung tissue injury, neutrophil infiltration, and inhibits inflammatory cytokine response through inhibition of NLRP3-signaling pathways

Of note, rapamycin treatment upregulated in vitro the expression of MITF and pro-melanogenic enzymes in melanocytes and melanoma cells (NCT05342519)