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Immunoprecipitation Hepatocytes were lysed in an immunoprecipitation (IP) buffer (150 mM NaCl), 50 mM Tris-HCl, 1% Nonidet P-40, pH 7.8, and a mammalian cell-specific protease inhibitor cocktail

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Materials and methods Compounds Details of compound sources, synthesis and validation are provided in the Chemistry Supplementary information

elik SB, Can H, Szmen MK, et al