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Eluted proteins were first concentrated using a 15-ml Amicon Ultra centrifugal filter (100,000 kDa cut-off) at 3,500 g and buffer exchanged into 20 mM Tris-HCl, 150 mM NaCl, 100 M ZnCl 2 and 10% glycerol, pH 7.5

Importantly, even though total lean mass decreased, the proportion of lean mass relative to total body weight actually increased in semaglutide patients

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doi: 10.1038/npp.2011.277 221 Martn-HernndezDTendilla-BeltrnHMadrigalJLMGarca-BuenoBLezaJCCasoJR