Vitamin B12 is very important for making megakaryocytes in the bone marrow
Reasons for increased needs in athletes: stress related to physical demands (Yes, training is stress on the body!) biological adaptations increased turnover (athletes use more vitamins) genetics reduced absorption Early detection of deficiency can be difficult to recognize because symptoms overlap with many other conditions and are normalized in athletes
Cells were lysed with RIPA lysis buffer (Strong) (P0013E-1, Beyotime) and proteins denatured with 4 sodium dodecyl sulphate (SDS) loading buffer (NP0007, Invitrogen)
Wajapeyee N, Serra RW, Zhu X, Mahalingam M, Green MR
vigorous shaking must be avoided
Shoulder injuries in strikers often respond well to modified pad work where partners adjust to reduce impact force