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After treatment with IGF-1, IGF-1R levels increased in membrane fraction of all the cell lysates compared to cytosolic fractions indicating IGF-1R migration to the membrane (Fig

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Western blotting Cells were lysed in RIPA lysis buffer with 2 mM phenylmethylsulfonyl fluoride, 1 mM sodium orthovanadate and protease inhibitor cocktail (Santa Cruz Biotech, Dallas, TX, sc-24948) for 30 min at 4 C and then centrifuged at 16,000 g for 15 min

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While randomized trials are the reference methodology for establishing treatment effectiveness, they leave many clinically relevant questions unanswered, which may delay access for new indications 29,30,31