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21502-04) was as follows: 94 C for 5 min, 35 cycles of melting at 95 C for 30 s, annealing at 63 C for 30 s, and extension at 72 C for 10 s, with final extension at 72 C for 5 min in the end

A PubMed (NIH) article regarding the effect of alcohol abuse and glutathione treatment states that "Glutathione (GSH) is a principal cellular scavenger of free radicals