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Under his HHS leadership, hes fired career scientists and staff at key public health agencies and installed like-minded allies into crucial roles

& Ellis, S

Gene expression analysis Total RNA isolation was performed with TRI reagent (Ambion) following the manufacturers protocol

[DOI] [PubMed] [Google Scholar] 139.Sun L., Wen S., Li Q., Lai X., Chen R., Zhang Z., Li D., Sun S

The blocked membranes were incubated with the specific primary antibodies diluted in 5% BSA (Sigma-Aldrich) with 0.05% sodium azide overnight on a shaking bed at 4 C