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To verify localization of CIPp-18 in cell membranes, cells were incubated with CIPp-18 bound to unlabeled albumin for 15 min at 37 C followed by an additional 15 min incubation in serum-free media containing 5 M Prodan, a cell membrane probe [37]

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Some practitioners prefer this approach for more severe injuries or when seeking maximum healing support

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Remarkably, the same narrow dose range proves effective whether counteracting dopamine depletion, serotonin syndrome, glutamate excitotoxicity, or GABA tolerance

A more dilute solution may be easier to measure accurately for small doses, while a more concentrated solution may be preferred if larger doses are needed