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Patients with Wilson's disease should not use any copper-containing therapy

doi: 10.1111/j.1471-4159.1991.tb02119.x

( mRNA quantification by quantitative real-time PCR (qRT-PCR) Total RNA was isolated using QIAzol-Reagent (Qiagen, Germany), based on modified Chomczyski & Sacchis method (Chomczynski and Sacchi, 2006), following the manufacturers protocol