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Plasma total cholesterol (Thermo Fisher Scientific, Infinity Total Cholesterol Reagent, TR13421), triglycerides (total glycerol and triglycerides) (Thermo Fisher Scientific, Infinity Total Triglycerides Reagent, TT22421), non-esterified fatty acids (NEFA) (Invitrogen, non-esterified free fatty acids (NEFA/FFA) Colorimetric Assay Kit, Thermo Fisher Scientific, EEA017), AST (Thermo Fisher Scientific, EEA003) and ALT (Thermo Fisher Scientific, EEA001) were quantified using enzymatic kits according to the manufacturers protocols

Gallbladder Issues: Can increase the risk of gallstones or gallbladder inflammation

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This approach minimises gastrointestinal side effects

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This has led to conceptualizing obesity as a chronic disease requiring ongoing management rather than a condition amenable to short-term cure