Butyrate promotes mitochondrial hyperfusion (via upregulating fusion proteins Opa1/Mfn2 and downregulating fission protein Fis1), enhances glucose-stimulated oxygen consumption and mitochondrial membrane potential, and restores redox homeostasis by increasing reduced glutathione (GSH) levels and the GSH/GSSG ratio
Following focusing, IPG strips were equilibrated for 15 minutes in equilibration buffer (30% v/v glycerol, 6 M urea, 1% w/v DTT) followed by 15 min in alkylating equilibration buffer (30% v/v glycerol, 6 M urea, 4% w/v iodoacetamide)
It helps reduce oxidative stress, which contributes to premature aging and cellular damage
Do not warm it beyond room temperature
doi: 10.1373/clinchem.2003.021634 8
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