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While there was also conservation in the TM bundle interactions when engaged by the four peptides, differences were identified in how each peptide uses different networks to facilitate G protein coupling

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Some users attempt to 'stack' Melanotan-1 with Melanotan-2 (a more potent MC1R agonist with additional melanocortin receptor activity) to accelerate pigmentation

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The most common reported effect is a 3060-second sting from the copper-peptide complex