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In contrast, evidence in patients with established HFrEF has been less consistent as small randomized trials reported neutral or potentially unfavorable findings in some patients with reduced ejection fraction [18, 19]

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The temperature of column oven was set at 40 C, and the injection volume was 1 L

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The extracted proteins were separated on BeyoGel Plus PAGE precast gels (P0468S, Beyotime Biotechnology) and transferred to polyvinylidene fluoride (PVDF) membranes

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Systemic copper absorption from creams and serums is minimal

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