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Liu J, Song L, Liu S, Zhao S, Jiang Q, Ding B

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The bacterial pellet from 100 ml of cultures was collected for each sample and resuspended in 20 mM potassium dihydrogen phosphate, sonicated for 10 min (200 W total power with 35% output, 2 s pulse, 3 s pause over ice), and centrifuged

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In addition, 24% in the surgical group developed kidney failure or died over the seven-year span of the study, compared to 44% in the non-surgical group

Sleep duration predicts cardiovascular outcomes: a systematic review and meta-analysis of prospective studies