Before (time point: 0 h) and after incubation for 12, 20 and 40 h at 24 C whilst shaking (150 rpm), aliquots (2.0 mL) were taken, autoclaved (15 min, 121 C), pellets collected by centrifugation (13000 rpm, 4 min) and extracted with EtOH (1 mL) by shaking overnight (15 h) at room temperature
However, no improvement was observed in performance times, blood lactate, pH, or base excess, indicating no ergogenic benefit for short, high-intensity anaerobic swimming (Hou et al
In his progression into Interventional Endocrinology, Dr
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