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Fluorescence (ex 545 nm/em 600 nm) was recorded with a microplate reader (CLARIOstar, BMG)

Because HepG2 cells are highly de-differentiated compared to normal hepatocytes in situ, and therefore are expected to have a less robust Met cycle and transsulfuration pathway than those found in normal liver, the GSH pool in normal liver could contain a substantially larger proportion of Met-derived S [115, 116, 118120]

Step 3: Divide your doses

its long-term complications include cirrhosis and HCC

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It travels to the nucleus and activates AMPK (AMP-activated protein kinase), the master regulator of cellular energy