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Figures 27 A & B indicate that those analogs that were previously found to block HGF dimerization were effective inhibitor of HGF/Met mediated cell scattering in MDCK cells, while those analogs with poor affinity for HGF were ineffective

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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions

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Furthermore, incomplete MOMP driven by mitochondrial adaptation and sublethal cytochrome c release has also been linked to the generation of BH3-mimetic drug-tolerant persister cells 76

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