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2.4 Safflower aqueous extract preparation For daily treatment, 5 g of the powdered flowers are macerated in 100 mL of heated distilled water for 1 h until they cool, and then used ( 2.5 HPLC analysis of safflower aqueous extract The polyphenols of safflower aqueous extract were determined using the HPLC system at the National Research Center (NRC) in Giza, Egypt, according to UV and diode array detection (DAD) methods ( 2.6 Collection of blood and samples After the experiment period (4 weeks) and following a 12-h fast, the rats were euthanized by CO 2 ( 2 flow by displacing 30%70% of the cage volume per minute until narcosis without removing them from their home to avoid stress caused by handling and then the narcotic rats were subjected to cervical dislocation

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Small-molecule oral GLP-1 RAs, such as orforglipron and danuglipron, are in development or early clinical trials

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However, postprandial hyperinsulinemia may exist before fasting hyperinsulinemia develops (31)