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Briefly, islets were handpicked, transferred to a petri dish containing 11 mL of KrebsRinger bicarbonate (KRB) buffer supplemented with 0.1% bovine serum albumin (BSA) and 3 mM glucose, and incubated at 37 C and 5% CO 2 for 60 min
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It is always a good idea to start with the recommended dose and consult with your healthcare provider if you have any underlying health conditions
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