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The treatment effect builds over the following three to four weeks as the body completes the clearance of the released fat

For Safranin O/Fast Green staining, slides were stained with hematoxylin (Mayer, Sigma) for 5 min, 0.005% Fast Green (Thermo Fisher Scientific, Pittsburgh, PA, United States) for 1 min, rinsed with 1% acetic acid, and stained with 0.1% Safranin O (Millipore, Billerica, MA, United States) for 20 min

GIP Receptor (GIPR) Glucose-dependent insulinotropic polypeptide is the other major incretin, released from intestinal K-cells

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Pharmacological, non-pharmacological and surgical interventions have been implemented in order to put an end to obesity and its complications, however, most of these therapies have failed because there is no sustained weight loss over time, so the use of GLP-1 receptor agonists has been implemented as effective drugs for weight reduction and modification of cardiovascular risk factors

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