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In the setting of HFpEF and diabetes, Hanff et al., using SomaScan assays and proteomics analysis of plasma from participants in the TOPCAT trial and the Penn Heart Failure Study, identified 10 proteins with significantly different expression in patients with HFpEF and diabetes.161 These proteins included fatty acid-binding protein, alpha-1-microglobulin/bikunin precursor, trafficking protein particle complex subunit 3, pigment epithelium-derived factor, tumour necrosis factor ligand superfamily member 15, ubiquitin-conjugating enzyme E2 G2, reticulon-4 receptor, insulin, cartilage intermediate layer protein 2 and apolipoprotein M
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After two weeks of treatment the rats were euthanized and the eyes were preserved with 4% paraformaldehyde in phosphate buffered saline (PBS)
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