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Clinical evaluation of melasma At the end of treatment period, hemi-MASI score was not significantly decreased on both right side (Glutathione gel treated side) ( P value = 0.1), and left side (Placebo treated side) ( P value = 0.3), with no significant difference between them ( P value=0.2) (Table 2)

H., Chahdoura, H., Barros, L., Dias, M

Step 5: Purification of compound of formula- 13 The crude compound of formula- 13 (72 gm) was dissolved in 50% methanol in water at 25-30C and sonicated