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Tissues were then incubated in 10, 20, and 30% sucrose in 1 PBS, embedded in OCT compound (Tissue-Tek, Sakura Finetek, 4583), snap frozen on dry ice, and then stored frozen at 80C

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846 Ferroptosis is present in the intestine during intestinal ischemia, along with increased expression of ACSL4 and decreased levels of FTH1 and GPX4, and inhibition of ACSL4 has protective effects on ferroptosis

The GshF low-level requirement was recommended from the global optimisation (Figure 6)