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There is a limit to drug accumulation because as the plasma concentration increased the amount of drug eliminated during the dosing interval will also increase as the rate of elimination is equal to the amount of the drug in the body multiplied by the rate constant for a first order elimination

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cerevisiae , were obtained from Twist Bioscience as DNA strands or subcloned into pET-28b(+) or pTwist Amp MC (Supplementary Tables 2 and 3)