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Initial investigations may include a pregnancy test (as unexpected ovulation can occur with weight loss), full blood count to assess for anaemia, thyroid function tests, and possibly prolactin levels if amenorrhoea is present

Body Composition Body composition was measured in mice fed ad libitum at 10-11 weeks of age using an EchoMRI-100 TM Body Composition Analyzer (EchoMRI LLC, Houston, TX)

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Ingredients Bulking agent cellulose, L-glutathione reduced, carriers calcium phosphate and cross-linked sodium carboxymethyl cellulose, lactose monohydrate, pomegranate peel extract (Punica L.), superoxide dismutase, maltodextrin, milk thistle seed extract (Silybum marianum L.), L-cysteine, anti-caking agents magnesium salts of fatty acids and silicon dioxide, glazing agent (neutral methacrylate copolymer, talc, propylene glycol, colorant carmine)