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Sample preparation and quantification for carnitine, TMAO, and -butyrobetaine of cardiovascular disease patient For optimal extraction, 180 L or 190 L of methanol spiked with 200 ng/mL of isotopically-labeled internal standards (d3-carnitine and d9-TMAO) was added to 20 L of plasma or 10 L of urine samples, respectively

Available from: Zarrinkoub R, Wettermark B, Wndell P, Mejhert M, Szulkin R, Ljunggren G et al (2013) The epidemiology of heart failure, based on data for 2.1 million inhabitants in Sweden

While these medications have shown some effectiveness in promoting weight loss, they generally produce modest results and are associated with side effects such as gastrointestinal discomfort and increased heart rate