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NOX, a vital contributor to ROS production, uses electrons from NADPH to generate O2

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RNA isolation and quantitative RT-PCR Samples for gene expression analysis were frozen and ground in liquid nitrogen and then total RNA purification, reverse transcription, and qRT-PCR were completed using one-step kit (TransGen Biotech)

We demonstrated that the in vitro kidney organoid model enabled the identification of a novel mechanism that may be used for the treatment of Fabry disease