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Immunofluorescence staining of oocytes After a brief rinse with PBS/0.05% polyvinylpyrrolidone (to prevent adhesion), the oocytes were permeabilized with 0.5% Triton X-100/PHEM for 5 min, fixed in 3.7% PFA/PHEM (18.14 g PIPES, 6.5 g HEPES, 3.8 g EGTA, 0.99 g MgSO4, pH 7.0 w/KOH) for 20 min, and washed three times (10 min each) with PBS/polyvinylpyrrolidone

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Studies on the quantitation and qualitative characterization of erythrocyte glutathione peroxidase

Clin Chim Acta 276:19 Sirtori LR, Dutra-Filho CS, Fitarelli D, Sitta A, Haeser A, Barschak AG, Wajner M, Coelho DM, Llesuy S, Bell-Klein A, Giugliani R, Deon M, Vargas CR (2005) Oxidative stress in patients with phenylketonuria

Samulak JJ, Sawicka AK, Samborowska E, Olek RA