The pharmacokinetic parameters assessed after multiple doses on Day 6 and Day 7 were: C max at steady state ( C max,ss ), t max at steady state ( t max,ss ), minimum concentration at steady state ( C min,ss ), AUC 0t at steady state (AUC 0t,ss ), AUC during the interval at steady state (AUC ,ss , calculated using the trapezoidal method), average concentration at steady state ( C ave,ss , calculated as AUC t,ss / t ), accumulation ratio (ARAUC, calculated as AUC ,ss /AUC 012 ) and peaktrough fluctuation [PTF, calculated as ( C max,ss C min,ss )/ C ave,ss 100)] of NAC in plasma, and Ae 0t at steady state (Ae 0t,ss ) of NAC in urine
Daniel O'Hara
Psychiatry 20 , 10571068 (2015)
If it produces 2-3 pounds like berberine alone, you pay $80-125 per pound lost
The substrate mix components are at the edge of solubility in DMSO, but once diluted in assay buffer, they are completely soluble
This leads to inaccurate, unreliable, and non-reproducible data, potentially invalidating the entire research project