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After 9 h of incubation, the 96-well plates were centrifuged and the supernatants were collected

Preventing moisture exposure requires attention at multiple points: During storage: keep vials in airtight containers, ideally with desiccant packs

All animal experiments were conducted in accordance with the NIH Guide for the Care and Use of Laboratory Animals or an equivalent standard that meets or exceeds the ethical and welfare requirements outlined in the NIH Guide

An upper lip lift shortens this vertical distance, re-establishing harmony among the nose, lips, and chin while restoring a natural curvature and renewed vitality to the smile

Complete appetite elimination for all seven days is not the goal, and achieving it would not necessarily improve outcomes