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Propidum iodide (PI) (Thermo Scientific) was used to selectively stain apoptotic cells

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The injections are doctor prescribed and administered into the deltoid muscle

StatPearls notes that high-dose oral B has been shown effective for maintenance, but recommendations are to always use the parenteral route in severe neurological manifestations

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The injection site should always be cleaned with an alcohol swab and allowed to dry before injecting