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Supernatants were assayed for total protein concentration with Bradford assay (BioRad 5000006) and 50100 g of protein was mixed with loading buffer and loaded in a 12 or 16% gradient gel (Thermo 1Fisher XP00162)

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It requires two copper ions at its active site to catalyse the conversion of tyrosine to DOPA and then to DOPAquinone the precursor of eumelanin

Ricketts, J., Jones, C.R., Happ, F., and Charman, T., Journal of Autism and Developmental Disorders , 2013, vol

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