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Vaughn AR, et al

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Over 1,000 types of E3 ligases have been identified, and this protein is primarily responsible for the specificity of ubiquitination[2]

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For H 2 O 2 assessment in whole cell, 1.510 4 cells were incubated in 100 M AmplexRed in Krebs-Ringer Phosphate buffer (145 mM NaCl, 5.7 mM Na 2 PO 4 , 4.86 mM KCl, 0.54 mM CaCl 2 , 1.22 mM MgSO 4 and 5.5 mM glucose, pH 7.4), and the luminescence recorded as we did for mitochondria