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The Ransod assay (RANDOX Laboratories, Crumlin, UK) was used to quantify the activity of superoxide dismutase (SOD), which is an antioxidant enzyme that protects cells against the pro-oxidant action of superoxide free radicals

[25] In dogs, it is estimated that 60% of cases are congenital with brachycephalic breeds being the most affected due to the oesophageal hiatus' cross-sectional area being larger than normocephalic and doliocephalic dogs

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