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This group includes liberated Hb and an enzyme called arginase 1 8

Vortex the solution for 30 s and perform ultrasonic treatment in iced water for 10 min

Slides were immersed in two changes of xylene for 10 dips each and mounted with Permount slide mounting medium (Thermo Fisher Scientific, catalog: SP15-100) using glass coverslips

doi: 10.7150/ijms.32222

Then a multivariable regression analysis was undertaken using the relevant variables associated with self-medication at the a-level of P 34 years remained significantly associated with self-medication (age groups: 3544 years OR 1.49, 95% CI = 1.01 to 2.20

doi: 10.1016/0196-9781(87)90163-x