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The bacterial pellet from 100 ml of cultures was collected for each sample and resuspended in 20 mM potassium dihydrogen phosphate, sonicated for 10 min (200 W total power with 35% output, 2 s pulse, 3 s pause over ice), and centrifuged

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Suitable injection sites include the abdomen (staying two fingers or a few inches away from the belly button), outer thighs, hips, upper buttocks, lower back, and backs of the arms