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For experiments in 384-well plates, 500 cells were seeded per well, and after 24 h, the medium was switched using a series of 4 aspirationwash steps with the desired final medium using Multidrop Combi (Thermo)

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Nerve pain (neuropathy) caused by chemotherapy : Acetyl-L-carnitine might worsen symptoms in some people with nerve pain caused by a class of chemotherapy drugs known as taxanes

However, this should only be done by or under the supervision of a licensed medical professional to ensure its done safely and effectively.