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For tissue validation, rectal samples from six experimental groups underwent immunohistochemistry (IHC) and immunofluorescence staining to examine p-p38, -catenin, and E-cadherin expression

Cells were harvested by centrifugation at 1000 x g for 3 min at room temperature and resuspended in 2 mL fresh HC -Ura-Leu medium containing 50 mM DTT to reduce all roGFP2 sensors before the start of the experiment

Others choose in-clinic treatment to manage overall expense while receiving the same formulation and protocol

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