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Stainless steel guide cannulas were bilaterally implanted into the DMV, the PVN or the other five brain regions following stereotaxic coordinates: the S1 coordinates: AP, 0.47 mm

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Beyond the gut, the microbiota influences distant organs through the production of bioactive compounds that enter the bloodstream, affecting metabolic functions, immune responses, and even neurobehavioral processes (Roman-Prez et al., 2021)

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Cecal samples were collected at euthanization, then flash-frozen and stored at 80 C