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A 4 mL blood sample from three healthy adult blood donors was collected via venipuncture in EDTA tubes in order to obtain PBMCs for cell viability assay

Growth Hormone Research Society perspective on the development of long-acting growth hormone preparations

Int J Geomate 13:121126

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The samples were desalted using a Zeba spin desalting column (Thermo Fisher Scientific) and 100 l of the desalted sample was combined with 100 l of 100 M NADPH