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Cells were cultured in Dulbeccos modified Eagles medium (DMEM) (Gibco, NY, USA) supplemented with 10 % foetal bovine serum (FBS) (Gibco, NY, USA) and were maintained in a humidified incubator at 37 C and 5 % CO 2

Organ- and function-specific anatomical organization and bioelectronic modulation of the vagus nerve

Furthermore, the authors reported that the MCF-7 breast cancer cell line shows an E2-dependent increase in its survival rate, which is abrogated by pre-treating them with the anti-estrogenic compounds ICI-182, 780, or raloxifene (Yu et al., 2012)

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Some early studies have shown higher pregnancy rates per embryo transfer in patients who used a GLP-1 medication before IVF compared with metformin alone, although the data remain preliminary and larger studies are still needed