Lipidomic profiling of CE and TG species was performed by mass spectrometry-based analysis
COA Batch: MPCJP001
To establish a direct link between METTL16 and CBS, we knocked down METTL16 in A549 and NCI-H23 cells, resulting in clear inhibition of CBS expression (Fig
GHB leaves the body quickly, usually within 6 to 12 hours, which is why it is rarely detected in standard drug tests
Although not previously reported, we also observed increases of lysine, dimethyl-arginine, and N-acetyl-(iso)leucine (Fig
Day 10 cultured SCD erythroblasts were treated with 75 M hemin (#51280, Sigma) (dissolved in 0.25 M NaOH then neutralized with HCL to pH7.4), 50 M dimethyl fumarate (#242926, Sigma) (dissolved in DMSO), 0.5 mM (2S)-Tetrahydro-5-oxo-2-furancarboxylic acid (TFMB-L2HG) (#SML2563, Sigma) (dissolved in DMSO), 0.5 mM S-(5-Adenosyl)-L-methionine iodide (SAM) (#A4377, Sigma) (dissolved in water), and cultured under hypoxia (1% O 2 ) using Heracell 150i incubator equipped with an Oxygen (O 2 ) sensor and Nitrogen (N2) gas supply and analyzed on day 12 of culture
