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A 2nd 100 l of 80 % methanol (80 C) was added to the pellet, the sample was vortexed, centrifuged at 13,000 g for 30 min at 4 C, and the 2nd 100 l of supernatant was saved

For the evaluation of the plasma and striatal NGF levels, ferritin and -tubulin, respectively, were used as loading controls

All bladder cancer cell lines were cultured in RPMI-1640 medium (11875093, Thermo Fisher, USA), supplemented with 10% fetal bovine serum (FBS) (10099-141, Gibco, USA), 100 U/mL penicillin, and 100 mg/mL streptomycin (15140122, Thermo Fisher, USA)

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Glutamine to proline conversion is associated with response to glutaminase inhibition in breast cancer

RLIP76 is the major ATP-dependent transporter of glutathione-conjugates and doxorubicin in human erythrocytes