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The suspension was freeze-thawed 3x at 80C, sonicated 3x 15 s then spun at 12 k RPM for 30 min to pellet the insoluble material

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Yes, our L-Carnitine is synthetically prepared in a laboratory, it is vegetarian since it isnt obtained from an animal source and contains no animal byproducts

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Then, the plate was centrifuged for 15 min at 4,000 g and 4 C (Eppendorf, 5810R) and replicated with the help of a liquid handler robotic station (Freedom Evo, TECAN) by transferring 20 l of supernatant into a new replica plate