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On the day of the experiment DMEM was removed, the cells were washed twice with Seahorse XF assay medium supplemented with 1 mM pyruvate and 5 mM glucose and incubated in it for 1 h in at 37C in a CO 2 -free incubator

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In a contemporary cohort of 16,492 patients with T2D and at high/very high CV risk participating in the SAVOR-TIMI 53 trial [6], CVD remained the leading cause of death and approximately one-third of all deaths were classified as sudden